ABSTRACT
@#To investigate the therapeutic effect and mechanism of sodium formononetin-3′-sulphonate (SFS) on collagen-induced rheumatoid arthritis in mice, C57 mice were induced with chicken type II collagen to establish a model of rheumatoid arthritis (collagen-induced arthritis, CIA), and were injected intraperitoneally with different doses of SFS (50,100,200 mg/kg). Body weight, food intake and foot swelling of all groups were observed during the experiment.After the treatment, TNF-α, IL-6, and IL-10 in the serum were detected with the CBA kit; NF-κB p65, p-NF-κB p65 (p-p65), TIPE2, PCNP and IκB-α in spleen tissue were determined by Western blot; the organ index, pathological changes of ankle joint cartilage tissue and the positive expression of NF-κB p65 in ankle joint tissue were also observed.The results showed that, compared with the model group, the body weight and food intake of mice in the treatment group increased, while the degree of foot swelling decreased; the expression levels of inflammatory factors TNF-α and IL-6 in serum decreased, while the expression of anti-inflammatory factor IL-10 increased; the expression levels of NF-κB p65, p-p65 and PCNP in spleen tissue decreased, while the expression of TIPE2 and IκB-α protein increased; the index of spleen and thymus of the CIA mice in the treatment group, the infiltration of inflammatory cells in the ankle joint, the destruction of synovial tissue and cartilage, and the positive expression of NF-κB p65 decreased.Among them, the high-dose group of SFS showed a better therapeutic effect.It is suggested that SFS has a therapeutic effect on CIA mice, and the mechanism may be achieved by regulating the NF-κB p65 signaling pathway and inhibiting the expression of inflammatory factors.
ABSTRACT
Viral infection is the main death cause of infectious diseases in China. The establishment of an animal model to mimic the progression of viral infectious diseases in humans is of great significance to the study of pathogenesis and prevention of viral infectious diseases. As a new animal model established and developed in recent years, tree shrew has showed obvious advantages and potentials compared with other non-human primates and mice which are commonly used as virus-infected animal models. In this paper, the biological advantages of tree shrew as a novel animal model of viral infectious diseases are summarized, including taxonomy, physiology and immunology. In addition, the latest application of tree shrew in the research of many viral infectious diseases such as hepatitis virus, herpes simplex virus, influenza virus and enterovirus infections are compared and summarized.
ABSTRACT
BACKGROUND:There are some controversies on the choice of immediate or delayed weight-bearing schemes of implants. OBJECTIVE: To explore the weight bearing scheme for Bego implants based on implant stability quotient (ISQ) measured by Osstel, and to analyze the factors which influence implants’ stability. METHODS:Seventy-four single Bego implants with ISQ≥ 50 in 62 patients were selected and randomly divided into test group (36 single Bego implants in 31 patients) and control group (38 single Bego implants in 31 patients). Patients in the test group were given immediate loading, and patients in control group were given delayed loading. Two groups’ ISQ of Bego implants after loading (1, 2, 3, 4, 6, 8 and 12 weeks) were compared, and factors which influence implants’ stability were analyzed. RESULTS AND CONCLUSION:The lowest ISQ for immediate loading of the test group appeared in 2-3 weeks after loading, and that of the control group appeared in 3-4 weeks. There was no statistical difference between two groups on ISQ of Bego implants after bearing (1, 2, 3, 4, 6, 8 and 12 weeks) (P > 0.05). Multiple regression analysis showed that the factors including age, types of osseous substance and implant length were positively related with stability of Bego implants, yet oral hygiene was negatively related with stability of Bego implants. Immediate loading and delayed loading have similar stability to single Bego implants with ISQ≥ 50 measured by Osstel, so individualized weight bearing scheme may be selected according to patient’s own condition under the guidance of Osstel, further to improve implants’ success rate after loading.